Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYLDYAATTPICEEALTVYQKLSMDMYGNASSLHDAGGKAKHILEYCREKIANIIGGEASGIYFTSGGTESNFLAI---------QSLLNGLPK--TKRHFITTAMEHQSIH-NCAAFLEQQGYDVTVIEPNEY-GLITEEILLTHIRPETGLVSIQHANSETGIIQPIQHLSSYLHNKG----------ILLHCDAVQTFGKIPINTKNLGVDALSMSSHKIHGPKGVGAVYIRPDVPWKPVYPLT---THEYGFRAGTVNVPGIGAFTAAAELIVSEMEKQISRNEALRTYFLDQIRIR-SLPVTLAADTSKAECLPHIIGCFF----HSFEGQYVMLECNRSNICISTGSACSAGYHGPSETMKALRKTEQEALQFIRISFGRHTTAEQLEQLLHTFTVLWEQKKGEFDIDRRIKANGRQQA 3A9X Chain:A ((20-430)) -VYMDYNATTPLEPEVIQAVTEAMKEAWGNPSSSYVAGRKAKDIINTARASLAKMIGGKPQDIIFTSGGTESNNLVIHSTVRCFHEQQTLQG---EGTRPHFITCTVEHDSIRLPLEHLVEDQVAEVTFVPVSKVNGQVEVEDILAAVRPTTCLVTIMLANNETGVIMPISEISRRIKALNQIRAASGLPRVLVHTDAAQALGKRRVDVEDLGVDFLTIVGHKFYGPR-IGALYVRGVGKLTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAADLVSENCETYEAHMRDIRDYLEERLEAEFGKRIHLNSRFPGVERLPNT--CNFSIQGSQLRGYMVLAQCQ--TLLASVGA------------------PVDVARNAVRLSVGRSTTRAEVDLIVQDLKQAVNQLEG----------------

General information: TITO was launched using: RESULT: Template: 3A9X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1896 -12356 -6.52 -35.10 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -6.52 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_3A9X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A9X-query.scw PDB file : Tito_Scwrl_3A9X.pdb: