Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYLDNSSTTKPYDEVLNVYEQTSSRYFGNPSSLHRYGAETEQLLQAAKNQIKRSLGLKKYDIVFTSGATEANN-------------AALKGAALSKIKTGKHIIATSIEHPSVTESLEQLKELFGFDITYLSVNE-DGFVSIEDLKAAIRPDTVLVSMMHVNNEVGSVQPIEAAGEVLKEHSNI---------LFHVDYVQGIYKVPLAIEKAGIDLCSISGHKFHGLKGTGALIVK---EGTRLIPLITGGSQQKGIRAGTEHTAGAVSLAKAINLASADFDTRLDTMTAVKELFMNRLSEIEGVVINT----PQMNSAPHIINFSVPGIKAEVLLHMLEEQDIFVSTTSAC-SAKEHKPSKVLLEMGKGEQIAGSSIRISLNYSQTSDVAEPFMNALRPGIKKLREMMR 3A9Z Chain:A ((20-428)) -VYMDYNATTPLEPEVIQAVTEAMKEAWGNPSSSYVAGRKAKDIINTARASLAKMIGGKPQDIIFTSGGTESNNLVIHSTVRCFHEQQTLQ--------TRPHFITCTVEHDSIRLPLEHLVEDQVAEVTFVPVSKVNGQVEVEDILAAVRPTTCLVTIMLANNETGVIMPISEISRRIKALNQIRAASGLPRVLVHTDAAQALGKRRVDVEDLGVDFLTIVGHKFYGPR-IGALYVRGVGKLTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAADLVSENCETYEAHMRDIRDYLEERLEAEFGKRIHLNSRFPGVERLPNTCNFSIQGSQLRGYMVLAQCQTLLASVGASCHSDHEDRPSPVLLSCGIPVDVARNAVRLSVGRSTTRAEVDLIVQDLKQAVNQL-----

General information: TITO was launched using: RESULT: Template: 3A9Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2030 5278 2.60 14.42 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : 2.60 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_3A9Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A9Z-query.scw PDB file : Tito_Scwrl_3A9Z.pdb: