Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEAVSPIRRFTVDG-VNVYYEHYQNPGRQTLVCVHGFLSSAFS---FRKVIPLLRDKYDIIALDLPPFGQSEKSRTFIYTYQNLAKLVIGILEHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLCSSGYLKRSHPTIIFGTHIPYFHLYIKRWLSKEGVMKNLLNV-------VHDKSLIDEEMID---GYGRPFQDEQIFKAMTRFIRHR--EGDLEPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHADLPNSVLYSLGQTGHLVPEERPELISEHIADFIK 2VF2 Chain:A ((30-307)) -ESTSRFAEVDVDGPLKLHYHEAGVGNDQTVVLLHGGGPGAASWTNFSRNIAVLARHFHVLAVDQPGYGHSDKRAEHGQFNRYAAMALKGLFDQLGLGRVPLVGNSLGGGTAVRFALDYPARAGRLVLMGPGG--------LSINLFAPDPTEGVKR-LSKFSVAPTRENLEAFLRVMVYDKNLITPELVDQRFALASTPESLTATRAMGKSFAGADFEAGMMWREVYRLRQPVLLIWGREDRVNPLDGALVALKTIPRAQLHVFGQCGHWVQVEKFDEFNKLTIEFL-

General information: TITO was launched using: RESULT: Template: 2VF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1512 -3655 -2.42 -13.95 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -2.42 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_2VF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VF2-query.scw PDB file : Tito_Scwrl_2VF2.pdb: