Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------MKIRKANINTQTGMITDVY--------LHENRKELRTLVAVPQLEWSTIISYEEDKA--TLPERLEASLRRHTEETPAGELAKKIIHWVTEM 2LAI Chain:A ((54-154)) SFGLGKAQDPLDKFFSKIIFSGKPIETSYSAKGIHEKIIEAHDLHVSKSKNAPIQYASVMEYLKKTYPGPDIERIVSTLERH-DEVGAKDLGAKLRDALDRQ

General information: TITO was launched using: RESULT: Template: 2LAI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 108 3807 35.25 47.00 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 35.25 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.335

(partial model without unconserved sides chains): PDB file : Tito_2LAI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LAI-query.scw PDB file : Tito_Scwrl_2LAI.pdb: