Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIVYAVNMGIVFIWSWFAKMCGGRDDSLATGYRPNKLLIWIPLASLVLVSGLRYRVGTDFQTYTLLYELAGDYQNVWQIFGFGTAKTATDPGFTALLWLMNFITEDPQIMYFTVAVVTYSFIMKTLADYGRPFELSVFLFLGTFHYYASFNGIRQYMVAAVLFWAIRYIISGNWKRYFLIVLVSSLFHSSALIMIPVYFIVRRKAWSPAIFGLSALFLGMTFLYQKFISVFVVVLENSSYSHYEKWLMTNTNGMNVIKIAVLVLPLFLAFCYKERLRSLWPQIDIVVNLCLLGFLFGLLATKDVIFARFNIYFGLYQMILVPYFVRIFDEKSNALIYIAIVVCYFLYSYLLMPVDSSVLPYRTIFSR 1JO6 Chain:A ((1-45)) ---------------------------------------------MFIWTSGRTSSSYRHDEKRNIYQKIRDHDLLDKRKTVTALKAGED-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1JO6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 20 -135 -6.75 -3.00 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.36 3D Compatibility (PKB) : -6.75 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.36 QMean score : -0.116

(partial model without unconserved sides chains): PDB file : Tito_1JO6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JO6-query.scw PDB file : Tito_Scwrl_1JO6.pdb: