Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGSTQLTGRVIF-KGDPGYT----EAIKN--WNPYVDVYPLVFVFAQNSYDVSNAIKWARENKVPLRVRSGRHALDKNLSVVS----GGIVIDVSDMNKVFLDEENAIATVQTGIPVGPLVKGL--ARDGFMAPFGDSPTVGIGGITMGGGFGVLSRSIGLISDNLLALKTVDAKGRIIHADQSHNEDLLWASRGGGGGNFGYNTQYTFKVHRAPKTATVFNIIWPW-EQLETVFKAWQKWAPFVDERLGCYLEIYSKING-LCHAEGIFLGSKTELIRLLKPLLHAGTP----TEADIKTLYYPDAIDFLD------PDEPIPGRNDQSVKFSSAWGHDFWSDEPISIMRKFLED--ATGTEANFFFINWGGAISRVPKDETAFFWRH-PLFYTEWTASWKNKSQEDSNLASVERVRQLMQPYVAG----SYVNVPDQNI---------------ENFGKEYYGANFARLREIKAKYDPENVFRFPQSIPPSR 3TSJ Chain:A ((18-495)) ----EIPPRLLYAKSSPAYPSVLGQTIRNSRWSSPDNVKPLYIITPTQVSHIQSAVVCGRRHSVRIRVRSGGHDYE-GLSYRSLQPETFAVVDLNKMRAVWVDGKARTAWVDSGAQLGELYYAIYKASPTLAFPAGVCPTIGVGGNFAGGGFGMLLRKYGIAAENVIDVKLVDANGK-LHDKKSMGDDHFWAVRGGGGESFGIVVAWQVKLLPVPPTVTIFKISKTVSEGAVDIINKWQVVAP----QLPADLMIRIIAQGPKATFEAMYLGT----CKTLTPLMSSKFPELGMNPSHCNEMSWIQSIPFVHLGHRDALEDDLLNRQNSFKPFAEYKSDYVYQPFPKTVWEQILNTWLVKPGAGIMIFDPYGATISATPESATPFPHRKGVLFNIQYVNYWFAPGAAAAPLSWSKDIYNYMEPYVSKNPRQAYANYRDIDLGRNEVVNDVSTYASGKVWGQKYFKGNFERLAITKGKVDPTDYFRNEQSIPP--

General information: TITO was launched using: RESULT: Template: 3TSJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2540 -13217 -5.20 -30.67 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -5.20 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.519

(partial model without unconserved sides chains): PDB file : Tito_3TSJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TSJ-query.scw PDB file : Tito_Scwrl_3TSJ.pdb: