Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFARDIGIDLGTANVLIHVKGKGIVLNEPSVVALDKNSGK--VLAVGEEARRMVGRTPGNIVAIRPLKDGVIADFEVTEAMLKHFINKLNVKGLFSKPRMLICCPTNITSVEQKAIKEAAEKSGGKHVYLEEEPKVAAIGAGMEIFQPSGNMVVDIGGGTTDIAVISMGDIVTSSSIKMAGDKFDMEILNYIKREYKLLIGERTAEDIKIKVATVFPDARHEEISI--RGRDMVSGLPRTITVNSKEVEEALRESVAVIVQAAKQVLERTPPELSADIIDRGVIITGGGALLNGLDQLLAEELKVPVLVAENPMDCVAIGTGVMLDNMDKLPKRKLS 4CZL Chain:A ((2-327)) -ISNDIAIDLGTANTLIYQKGKGIVLNEPSVVALRNVGGRKVVHAVGIEAKQML------MEAIRPMRDGVIADFEVAEEMIKYFIRKVHNRKGSGNPKVIVCVPSGATAVERRAINDSCLNAGARRVGLIDEPMAAAIGAGLPIHEPTGSMVVDIGGGTTEVAVLSLSGIVYSRSVRVGGDKMDEAIISYMRRHHNLLIGETTAERIKKEIGTARAPADGEGLSIDVKGRDLMQGVPREVRISEKQAADALAEPVGQIVEAVKVALEATPPELADDIADKGIMLTGGGALLRGLDAEIRDHTGLPVTVADDPLSCVALGCGKVLEH----------

General information: TITO was launched using: RESULT: Template: 4CZL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1837 -1778 -0.97 -5.63 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -0.97 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_4CZL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CZL-query.scw PDB file : Tito_Scwrl_4CZL.pdb: