Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIKAQIGMVMNLDKCIGCHTCSVTCKNTWTNRSGAEYMYFNNVETKPGIGYPKQWEDQDKYKGGWTLKKGKLELKSGSKTNRLAGLFYNPNQPSIDDYYEPWNYDYETLTNSPQKKHQPVA-RPKSSLTGDFMNIEWGPNWEDDLAGGHITGLEDPNVQKMEESIKTEFDDVFMMYLPRICEHCINPACVSSCPSGAMYKREEDGIVLVDQNACRSWRYCVSSCPYKKVYFNWQTNKAEKCTLCFPRLEAGLPTICSETCVGRIRYLGVMLYDADKVEEAASVENEKDLYHSQLDVFLDPNDPEVAKLAKEQGIPAEWIEAAQQSPIYKMIIDWKIALPLHPEYRTLPMVWYIPPLSPIMNLFEGKGSRQTAEDIFPAIDQMRIPIDYLAQLLTAGDTDHIRSTLKKMSVMRQYMRAVQTNKSIDPELISSVGLTEQQIEDMYRLLAIAKYDDRFVIPSSHREEVSDLYAEQGSCGLSFSGGPGSCF 2IVF Chain:B ((16-330)) ---KRQLVTVIDLNKCLGCQTCTVACKNIWTKRPGTEHMRWNNVTTYPGKGYPRDYE-----------RKGGGFLRGEPQPGVL---------PTLIDSGDDFQFNHKEVFY--EGKGQTVHFHPTSKSTGK--DPAWGYNWDEDQGGG-------------------KWPNPFFFYLARMCNHCTNPACLAACPTGAIYKREDNGIVLVDQERCKGHRHCVEACPYKAIYFNPVSQTSEKCILCYPRIEKGIANACNRQCPGRVRAFG----------------------------YLDDTTSHVHKLVKK----------------------WKVALPLHAEYGTGPNIYYVPPM----------GARGFGED-GEITDKTRIPLDVLEGLFGP----EVKRVLAVLHTERENMRAGRGSELMD--------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2IVF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1674 -43424 -25.94 -138.29 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -25.94 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.310

(partial model without unconserved sides chains): PDB file : Tito_2IVF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IVF-query.scw PDB file : Tito_Scwrl_2IVF.pdb: