Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLLAIYPGSIVSEELCIREGCLCFFDESNQRYISIPKTEISEKEVLVLQSFLTPADEGNQLSMKSPEENKWFSFLFSRGELPAYIKKRTRFVHFHLFGKIERTSFTEAVRHFWPVSFVIVWIHEDRGVIVEQESEAAAEKDELESLAKVLESDFYFSVRFYAGRFYEPDECLRKHYAREQAYFLFAEKRLPQVQSVTFEMIFPFLLLETEKEKLETLLSEEAELLFGSESELRKTIKLFIENNSNVTLTAKKLHLHRNSLQYRIDKFIERSGIDIKSYKGALLAYFICLQNESSE 3ONQ Chain:B ((201-247)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TVSTFLKYGSSLENTAKELNVHPNTVRYRLKRAAETTGWDATDPRDA--------------

General information: TITO was launched using: RESULT: Template: 3ONQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 150 -8974 -59.83 -190.94 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.54 3D Compatibility (PKB) : -59.83 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_3ONQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ONQ-query.scw PDB file : Tito_Scwrl_3ONQ.pdb: