Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEWNKTKSIFIVAFLILDIFLGYQFFQKWQATGKEYEVIKNDVEHDMKADHITYEGLNKEATEGYRITANQKSFSKEEIEALKDQKPLMDMPSDDHKVTSLKMKFANPIALSKKDIEDDAQALVSSKIQDGEKYKLWKVD----KSKKEIIFFQTYEGHYIYQKTDNPSNMIGQVVLHLNGKNEVVSYDQTTLETFKQI----QKESLITEMDAVELLYYQNQL---------------------KEYSTVKSCKFGYVAQYPLTSTQVLAPVWRITVEYEKKVNGEKKTVQEYFTVNALESTILDTDQ 4K90 Chain:B ((35-207)) -----------------------------------------------------------------------------------------------------------------QSYVEVATQHVKMIAP-DATFRVVDDHYVGDNGVAHVHFRQTANGLDIDN---------ADFNVNVGKDGKVFSYGNSFYTGQIPSSAALTKRDFSDPVTALKGTTNTLQLPITVDSASSESTKESYVFKGVSGTVSDPKAKLVYFVKD----DGTLALAWRVETDIDS---N--W---LLTYIDAKSGEEIH---

General information: TITO was launched using: RESULT: Template: 4K90.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 545 16244 29.80 114.39 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : 29.80 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.512

(partial model without unconserved sides chains): PDB file : Tito_4K90.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K90-query.scw PDB file : Tito_Scwrl_4K90.pdb: