Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKFKKNFLVGLSAALMSISLFSATASAASTDYWQNWTDGGGIVNAVNGSGGNYSVNWSNTGN--FVVGKGWTTGSP-----FRTINYNAGVWAPNGNGYLTLYGWTRSPLIEYYVVDSWGTYRPTGTY-KGTVKSDGGTYDIYTTTRYNAPSIDGDRTTFTQYWSVRQSKRPTGSNATITFSNHVNAWKSHGMNLGSNWAYQVMATEGYQSSGSSNVTVW 1H4G Chain:A ((3-196)) --------------------VTDNSIGNHDGYDYEFWKDSGGSGTMILNHGGTFSAQWNNVNNILFRKGKKFNETQTHQQVGNMSINYGA-NFQPNGNAYLCVYGWTVDPLVEYYIVDSWGNWRPPGATPKGTITVDGGTYDIYETLRVNQPSIKGIA-TFKQYWSVRRSKR-TS--GTISVSNHFRAWENLGMNMGK-MYEVALTVEGYQSSGSANVYS-

General information: TITO was launched using: RESULT: Template: 1H4G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1029 32716 31.79 175.89 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 31.79 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.486

(partial model without unconserved sides chains): PDB file : Tito_1H4G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H4G-query.scw PDB file : Tito_Scwrl_1H4G.pdb: