Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILVLAVHPHME--TSVVNKAWAEELSKH-DNITVRDLYKEY--PD-----------------------------EAIDVAKEQQLCEEYDRIVFQFPLYWYSSPPLLKKWQDLVLTYGWAFGSE------------GNALHGKELMLAVSTGSEAEKYQAGGANHYSISELLKP-FQATSNLIGMKYLPPYVFYGVNYAAAEDISHSAKRLAEYIQQPFV 4GI5 Chain:A ((23-241)) MKVLLIYAHPEPRSLNGALKNFAIRHLQQAGHEVQVSDLYAMRWKAGYDADDSGAPPVGEFWRPTLDSKQAFAQGTQSADIVAEQEKLLWADTVIFQFPLWWFSMPAIMKGWIDRVYAWGFAYGVGEHSDRHWGDRYGEGTFVGKRAMLIVTAGGWAEHYSPRGIN-GPIDDILFPIQHGMLFYPGFEVLPPLVFYRTDKTDAGQFADQCAALAERLDTL--

General information: TITO was launched using: RESULT: Template: 4GI5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 682 -9675 -14.19 -56.25 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -14.19 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.561

(partial model without unconserved sides chains): PDB file : Tito_4GI5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GI5-query.scw PDB file : Tito_Scwrl_4GI5.pdb: