Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYRILVVEDDEDIGDLLEESLTRAGYEVLRAKDGKRALQLVN-DSLDLVILDIMMPGISGIETCQHIRK-SSNVPILFLTARSSTLDKTEGLLAGGDDYMTKPFSEEELHARVIAQLRRYTIYQEKKEQEETFLIGGKLRVSEEFNEVWKEEKQIKLSDLEYRILKLLMNKRNKIFSAQNIYESVWGQPYFYCSNNTVMVHIRKLRSKIEDDPARPVYIKTEWGRGYRFGAS 4S04 Chain:A ((2-218)) ---KILVIEDDALLLQGLILAMQSEGYVCDGVSTAHEAALSLASNHYSLIVLDLGLPDEDGLHFLSRMRREKMTQPVLILTARDTLEDRISGLDTGADDYLVKPFALEELNARIRALLRRHNNQGDN------EISVGNLRLNVTRRLVWLGETALDLTPKEYALLSRLMMKAGSPVHREILYNDIYSG-DNEPATNTLEVHIHNLREKIGK-----SRIRTVRGFGYMLAN-

General information: TITO was launched using: RESULT: Template: 4S04.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1005 -23541 -23.42 -109.49 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -23.42 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_4S04.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4S04-query.scw PDB file : Tito_Scwrl_4S04.pdb: