Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKILLVYATMSGNTEAMADLIEKGLQEALAEVDRFEAMDIDDAQLFTDYDHVIMGTYTWGDGDLPDEFLDLVEDMEEIDFSGKTCAVFGSGDTA-Y-EFFCGAVDTLEAKIKERGGDIVLPSVKIENN--------------------PE--GEEEEELINFGRQFAKKSGCAV 1CZN Chain:A ((1-168)) -AKIGLFYGTQTGVTQTIAESIQQEFGGES-IVDLNDIANA-DASDLNAYDYLIIGCPTWNVGELQSDWEGIYDDLDSVNFQGKKVAYFGAGDQVGYSDNFQDAMGILEEKISSLGSQTVGYW-PIEGYDFNESKAVRNNQFVGLAIDEDNQPDLTKNRIKTWVSQLKSEFG---

General information: TITO was launched using: RESULT: Template: 1CZN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 692 -84603 -122.26 -587.52 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -122.26 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.607

(partial model without unconserved sides chains): PDB file : Tito_1CZN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CZN-query.scw PDB file : Tito_Scwrl_1CZN.pdb: