Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQFDKQYNSIIKDIINNGISDEEFDVRTKWDSDGTPAHTLSVISKQMRFDNSE-VPILTTKKVAWKTAIKELLWIWQLKSNDVNDLNMMG-VHIWDQ----WKQEDGTIGHAYGFQLGKKN------RSLNGEKVDQVDYLLHQLKNNPSSRRHITMLWNPDELDAMALTPCVYETQWYVKHGKLHLEVRARSNDMALGNPFNVFQYNVLQRMIAQVTGYELGEYIFNIGDCHVYTRHIDNLKIQMEREQFEAPELWINPEVKDFY---DFTIDDFKLINYKHGDKLLFEVAV 1BKP Chain:A ((2-279)) -TQFDKQYNSIIKDIINNGISDEEFDVRTKWDSDGTPAHTLSVISKQMRFDNS-EVPILTTKKVAWKTAIKELLWIWQLKSNDVNDLNMM-GVHIWDQWKQE----DGTIGHAYGFQLGKK-NRSLNG-----EKVDQVDYLLHQLKNNPSSRRHITMLWNPDELDAMALTPCVYETQWYVKHGKLHLEVRARSNDMALGNPFNVFQYNVLQRMIAQVTGYELGEYIFNIGDCHVYTRHIDNLKIQMEREQFEAPELWINPEVK---DFYDFTIDDFKLINYKHGDKLLFEVAV

General information: TITO was launched using: RESULT: Template: 1BKP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1242 -1551 -1.25 -5.90 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.96 3D Compatibility (PKB) : -1.25 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.96 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_1BKP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BKP-query.scw PDB file : Tito_Scwrl_1BKP.pdb: