Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEAVSPIRRFTVDGVNVYYEHYQNPG-RQTLVCVHGFLSSAFSFRKVIPLLRDKYDIIALDLPPFGQSEKSRTFIYTYQNLAKLVIGILEHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLCSSGYLKRSHPTIIFG-T-----HIPYFHLYIKRWLSKEGVMKNLLNVVHDKSLIDEEMIDGYGRPFQDEQIFKAMTRFIR------------HREGDLEPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHADLPNSVLYSLGQTGHLVPEERPELISEHIADFIK 3WI7 Chain:A ((22-299)) ------AYDVPAFGLQI---HTVEHGSGAPIVFLHGNPTSSYLWRHIFRRLHGHGRLLAVDLIGYGQSSKPD-IEYTLENQQRYVDAWFDALDLRNVTLVLQDYGAAFGLNWASRNPDRVRAVAFFEPVLRNIDSVDLSPEFVTRRAKLRQPGEGEIFVQQENR-FLTELFPWFFL--TPLAPEDLRQYQTPFPTPHSRKAILAGPRNLPVDGEPASTVAFLEQA-VNWLNTSDTPKLLLTFKPGFLLTDAILKWSQVTIRNLEIEAAGAGIHFVQEEQPETIARLLDAWLT

General information: TITO was launched using: RESULT: Template: 3WI7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1335 -31507 -23.60 -121.65 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -23.60 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_3WI7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WI7-query.scw PDB file : Tito_Scwrl_3WI7.pdb: