Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAHTEQYRFPKDFWWGSSASATQMEGAADRDGKGQNIWDYWFEKEPHRFFDHVGPADTSQ----FYDNYKEDIRLMKELGHNSFRMSISWSRLIPNGTGE-INDKAADFYNNVIDELIANGIEPFVNLFHFDMPMALQ--KIGGWVNRE-TVDAYENYARTCFRLFGGRVKKWFTHNEPIVPVEGGYL---------YDFHYPNKVDFKEAVQVGFHTMLSSARAIQAYREM--KQDGKIGIILNLTPSYPRSSH-PADVKAGEIADAFFNRSFLDPSVKGEFPKELVDILKHEGFMPDYNAEDLDIIKKNTVDLLGVNYYQPRRVKAKEHLPNPDAPFLPDRYFDPYVMPGRKMNPHRG-----WEIYEKGVYDILINLKENYGNIECFISENGMGVE-GEERFRDEQGIIQDDYRIEFIKEHLKWIHRAIQEGSNVKGYHLWTFMDNWSWTNAYKNRYGFVSVNLEKDGERTVKKSGKWFKEVAEHSGF---- 1E4I Chain:A ((1-447)) -TIFQ---FPQDFMWGTATAAYQIEGAYQEDGRGLSIWDTFAH-TP----GKVFNGDNGNVACDSYHRYEEDIRLMKELGIRTYRFSVSWPRIFPNGDG-EVNQKGLDYYHRVVDLLNDNGIEPFCTLYHWDLPQALQDA--GGWGNR-RTIQAFVQFAETMFREFHGKIQHWLTFNEPWCIAFLSNMLGVHAPGLT---------NLQTAIDVGHHLLVAHGLSVRRFRELGT--SGQIGIAPNVSWAVPYS-TSEEDKAACARTISLHSDWFLQPIYQGSYPQFLVDWFAEQGATVPIQDGDMDIIG-EPIDMIGINYYSMSVNRFNP---------EAGFLQSEEIN------MGLPVTDIGWPVESRGLYEVLHYLQ-KYGNIDIYITENGACINDE----VVN-GKVQDDRRISYMQQHLVQVHRTIHDGLHVKGYMAWSLLDNFEWAEGYNMRFGMIHVDFRT-QVRTPKQSYYWYRNVVSNNWLETRR

General information: TITO was launched using: RESULT: Template: 1E4I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2408 -214801 -89.20 -515.11 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -89.20 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.512

(partial model without unconserved sides chains): PDB file : Tito_1E4I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E4I-query.scw PDB file : Tito_Scwrl_1E4I.pdb: