Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADLKTQILDAYNFRHATKEFDPNKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWG-AQKQLPTASHFVLILARTAKDIKYNADYIKRHLKEVKQMPQDVYEGYLSKTEEFQKNDLHLLES---DRTLFDWASKQTYIALGNMMTAAAQIGVDSCPIEGFQYDHIHRILEEEGLLENGSFDISVMVAFGYRVRDPRPKTRSAVEDVVKWV 4QLX Chain:A ((10-218)) --LVNNDLADVMFNRHSVRQFDPNVKIGRDELQKMIAEAATAPSACNLQSWHFVVVDTPEAKAKFKQAVMKFNYPQVDSASAIVFIAGDTQSHY-VYRDVWNKVYEDG-NITKE----RLDQILGTFL----PLYENATPDFLKFDATIDCSVVGMQLLLVARAHGYDANAFSGIDFEKMIPTLG----LDPKRYVPVMGIAIGKAAQEPLHTTRYDAKTQTDFL

General information: TITO was launched using: RESULT: Template: 4QLX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 860 -106568 -123.92 -519.84 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -123.92 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_4QLX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QLX-query.scw PDB file : Tito_Scwrl_4QLX.pdb: