Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIAISGGG-TGGHIYPALAFIKEVQRRHPNVEFLYI--GTENGLEKKIVERENIPFRSIEITGFKRKLSFENVKTVMRFLKGVKKSKSYLAEFKPDAVIGTGGYVCGPVVYAAAKMG--IPTIVHEQNSLP-GITNKFLSKYVNKVAICFEEAKSHFP-----SEKVVFTGNPRASEVVSIKTGRSLA-E-FGLSEDKKTVLIFGGSRGAAPINRAVIDMQDVLKT-------RDYQVLYITGEVHYEKVMNELKSKGAADNMVTKPFLHQMPEYLKAIDVIVARAGATTIAEITALGIPSVLIPSPYVTANHQEVNARSLGQHDAAIVLKETELSGEKLIEALDRIVLN-EQTLKEMSERTKSLGVPDAAARLYSVLEELKK 4X1T Chain:A ((6-383)) KKVLILMSDTGGGHRASAEAIRAAFN-QEFGDEYQVFIT-------------------------------------------------------QPDIIISVHPLMQHVPLRVLRSKGLLKKIVFTTVITDLSTCHPTWFHKLVTRCYCPSTEVAKRAQKAGLETSQIKVYGLPVRPSFVKPVRPKVELRRELGMDENLPAVLLMGGGEGMGPI-EATARALADALYDKNLGEAVGQ-VLIICGRNKKLQS--KLSSLDWKIPVQVKGFITKMEECMGACDCIITKAGPGTIAEAMIRGLPIILNGYIAG---QEAGNVPYVVENGCGKFS--KSPK--EISKIVADWFGPASKELEIMSQNALRLAKPEAVFKIVHDMHEL--

General information: TITO was launched using: RESULT: Template: 4X1T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1716 -205300 -119.64 -698.30 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -119.64 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_4X1T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X1T-query.scw PDB file : Tito_Scwrl_4X1T.pdb: