Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGIAWMIVFCEIAFWVVIVLGLAVRYVFKRHTLGLLFLALTPVIDLILLAATGVDLYRGASATAAHGIAAVYIGISIAYGKQMIQWADEKFQYYVTKKGTKP-----LKRFGMDHAKHGAKGWLRHVLAYLIGAGLLAGMIYFINDSS-RTEALSGILKLWTVIIGIDF-LITASYFIWPKKEKASANLRS 1FLC Chain:B ((4-165)) ----------------------------IDDLIIGVLFVAIVETGIGGYLLGSRKESGGGVTKESAEK-GFEKIGNDIQILKSSINIAIEKLNDRISHDEQAIRDLTLEIENARSEALLGELGIIRALLVGNISIGLQESLWELASEITNRAGDLAVEVSPGCWIIDNNICDQSCQNFIFKFNETAPVPTI-

General information: TITO was launched using: RESULT: Template: 1FLC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 383 -45557 -118.95 -293.91 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -118.95 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.112

(partial model without unconserved sides chains): PDB file : Tito_1FLC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FLC-query.scw PDB file : Tito_Scwrl_1FLC.pdb: