Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKWNPLIPFLLIAVLGIGLTFFLSVKGLDDSREIASGGESKSAEKKDANASPEEIY-KANCIACHGENYEG-VSGPSLKGVGDKKDV-----AEIKTKIEKGG------NGMPSGLVPADKLDDMAEWVSKIK 3WFD Chain:C ((45-133)) --------------------------------------------MSAAVVRGKLVWEQNNCVGCHTLLGEGAYFAPELGNVVGRRGGEEGFNTFLQAWMKIQPLNVPGRRAMPQFHLSEGQVDDLAEFLKWSS

General information: TITO was launched using: RESULT: Template: 3WFD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 163 -3795 -23.28 -49.93 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain C : 0.61 3D Compatibility (PKB) : -23.28 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.355

(partial model without unconserved sides chains): PDB file : Tito_3WFD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WFD-query.scw PDB file : Tito_Scwrl_3WFD.pdb: