Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTLVIVIHPNLE--TSVVNKTWMNRLKQE-KDITVHDLYGEY--PN------------------------------FIIDVEKEQQLLLDHERIVFQFPMYWYSSPALLKQWEDDVLTHGWAYGTG----GTKLHGKELLLAISLGAQESDYQAGGEYNITISELIR-PFQVTANYIGMRFLPAFTQYGTLHLSKEDVKNSAERLVDYLKAEH 4FGL Chain:A ((6-216)) KKVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNFEPRATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIFQFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTGGTAEMYTKTGV-NGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEERKGMVAAWSQRLQT--

General information: TITO was launched using: RESULT: Template: 4FGL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 721 2347 3.25 13.72 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 3.25 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.549

(partial model without unconserved sides chains): PDB file : Tito_4FGL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FGL-query.scw PDB file : Tito_Scwrl_4FGL.pdb: