Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYRILVVEDDEDIGDLLEESLTRAGYEVLRAKDGKRALQLVN-DSLDLVILDIMMPGISGIETCQHIRKSSNVPILFLTARSSTLDKTEGLLAGGDDYMTKPFSEEELHARVIAQLRRYTIYQEKKEQEETFLIGGKLRVSEEFNEVWKEEKQIKLSDLEYRILKLLMNKRNKIFSAQNIYESVWGQPYFYCSNNTVMVHIRKLRSKIEDDPARPVYIKTEWGRGYRFGAS 3Q9S Chain:A ((5-212)) ---RILVIEDDHDIANVLRMDLTDAGYVVDHADSAMNGLIKAREDHPDLILLDLGLPDFDGGDVVQRLRKNSALPIIVLTARDTVEEKVRLLGLGADDYLIKPFHPDELLARVKVQLRQRTSESL---------SMGDLTLDPQKRLVTYKGEELRLSPKEFDILALLIRQPGRVYSRQEIGQEIWQG-RLPEGSNVVDVHMANLRAKLRDLD-GYGLLRTV----------

General information: TITO was launched using: RESULT: Template: 3Q9S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 895 -36211 -40.46 -174.93 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -40.46 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_3Q9S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3Q9S-query.scw PDB file : Tito_Scwrl_3Q9S.pdb: