Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEAVSPIRRFTVDGVNVYYEHYQNPG-RQTLVCVHGFLSSAFSFRKVIPLL-RDKYDIIALDLPPFGQSEKSRTFIYTYQNLAKLVIGILEHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLCSSGYLKRSHPTIIFG-TH-IPYFHLYIKRWLSKEGV---MKNLLNVVHD---KSLIDEEMIDGYGRPFQDEQIFKAMTRF-----------IRHREGDLEPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHADLPNSVLYSLGQTGHLVPEERPELISEHIADFIK 3U1T Chain:A ((12-294)) -------RTVEVEGATI---AYVDEGSGQPVLFLHGNPTSSYLWRNIIPYVVAAGYRAVAPDLIGMGDSAKPD-IEYRLQDHVAYMDGFIDALGLDDMVLVIHDWGSVIGMRHARLNPDRVAAVAFMEALVPPALPMPSYEAMGPQLGPLFRDLRTADVGEKMVLDGNFFVETILPEMGVVRSLSEAEMAAYRAPFPTRQSRLPTLQWPREVPIGGEPAFAEAEVLKNGEWLMASPIPKLLFHAEPGALAPKPVVDYLSENVPNLEVRFVGAGTHFLQEDHPHLIGQGIADWLR

General information: TITO was launched using: RESULT: Template: 3U1T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1383 -32474 -23.48 -123.95 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -23.48 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_3U1T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3U1T-query.scw PDB file : Tito_Scwrl_3U1T.pdb: