Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHQIVTTQYGKVKGTTENGVHKWKGIPYAKPPVGQWRFKAPEPPEVWEDVLDATAYGSICPQPSDLLSLS----YTELPRQSEDCLYVNVFAPDTP-SKNLPVMVWIHGGAFYLGAGSE-PLYDGSKLAAQGEVIVVTLNYRLGPFGFLHLSSFN--EAYSDNLGLLDQAAALKWVRENISAFGGDPDNVTVFGESAGGMSIAALLAMPAAKGLFQKAIMESGASRTMTK-E--QAASTSAAFLQVLGINEGQLDKLHTVSAEDLLKAADQLRIAEKENIFQLFFQPALDPKTLPEEPEKAIAEGAASGIPLLIGTTRDEGYLFFTPDSDVHS-QETLDAALEYLLGK---PLAEKVADLYPRSLESQIHMMTDLLFWRPAVAYASAQSHY-APVWMYRFDWHPKKPPYNKAFHALELPFVFGNLDGLERMAKAEITDEVKQLSHTIQSAWITFAKTGNPSTE-AVNWPAYHEETRETLILDSEITIENDPESEKRQKLFPSKGE 5A2G Chain:A ((9-500)) -NLPVVETKAGKLRGYQWEGTYIFKGIRYARA----NRFQLPEEVEPWEGVKEAASYGFVCPMLTRDHPQGELLVPHRYWPQDEDCLSLNIWSQSLDRSAKKPVMFWIHGGAFSMGSSIEQKAYNGENMSRYGDVVVVTVNHRLNILGYLDLSPYGERYAGSANAGQADLVAALKWVRDNIEAFGGDPDNVTIFGQSGGGMKVSGLMQTPEADGLFHRAMIMSGVAGDVLPYSTGDSRPLIQAMLKELGLAEQEAGRLETVPYYDLAAAYNRVSPAIARAGGYIGCTPRPDDFYKGEGPAVGFT-DHAKTIPVMVGTVFGEFAMMPLPFNKETISEAELDEILDKRFQGHGKELKTVFAEAYPGK-SPVDLLTLDTIFRGPTKEFVRSLAAAGGSVYSYLFALEFPYQNQKTAWHCSDIPFIFHNTELVPVT--NI-PEISDKLEKQMFDAVIHFVETGDPNHLGIPQWPVSTEDREATMIFDRVCTVRFNFDDYLLELYKK----

General information: TITO was launched using: RESULT: Template: 5A2G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2819 -3324 -1.18 -7.00 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -1.18 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.577

(partial model without unconserved sides chains): PDB file : Tito_5A2G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5A2G-query.scw PDB file : Tito_Scwrl_5A2G.pdb: