Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGYLHDAFSLKGKTALVTGPGTGIGQGIAKALAGAGADIIGTSHTSSLSETQQLVEQEGRIFTSFTLDMSKPEAIKDSAAELFENRQIDILVNNAGIIHREKAEDFPEENWQHVLNVNLNSLFILTQLAGRHMLKR-GHGKIINIASLLSFQGGILVPAYTASKHAVAGLTKSFANEWAASGIQVNAIAPGYISTANTKPIRDDEKRNEDILKRIPAGRWGQADDIGGTAVFLASRASDYVNGHILAVDGGWLSR 4HP8 Chain:A ((4-246)) ------PFSLEGRKALVTGANTGLGQAIAVGLAAAGAEVVCAARRAPD-ETLDIIAKDGGNASALLIDFADPLAAKDSFTD----AGFDILVNNAGIIRRADSVEFSELDWDEVMDVNLKALFFTTQAFAKELLAKGRSGKVVNIASLLSFQGGIRVPSYTAAKHGVAGLTKLLANEWAAKGINVNAIAPGYIETNNTEALRADAARNKAILERIPAGRWGHSEDIAGAAVFLSSAAADYVHGAILNVDGGWLA-

General information: TITO was launched using: RESULT: Template: 4HP8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1373 -75516 -55.00 -312.05 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.89 3D Compatibility (PKB) : -55.00 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_4HP8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HP8-query.scw PDB file : Tito_Scwrl_4HP8.pdb: