Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGNTRKKVSVIGAGFTGATTAFLIAQKELA-DVVLVDIPQLENPTKGKALDMLEASPVQGFDAKITGTSNYEDTAGSDIVVITAGIARKPGMSRDDLVSTNEKIMRSVTQEIVKYSPDSIIVVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTFVAEELNLSVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLETLIP-------KERIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTEMVEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIVGGNGLEQIIELELTDYERAQLNKSVESVKNVMKVLS 4M1Q Chain:A ((25-335)) --TKTSRVVIIGTGAVGSSYAFSMINQNVTDEMVLIDLD-KR-KTEGDAMDLNHGI-PFGAPTKVW-AGDYGDCKSADIVVITAGAAQKPGETRLDLVEKNANIFKGIVDQVMGSGFNGIFIIATNPVDVLAYATWKFSGLPKERVIGSGTILDTARFRFLLSEYFDIDVRNIHGYIMGEHGDTELPVWSQTRIGSEPISRYMDKYKPDGSNKDLDEIFVNVRDAAYHIIERKG--ATHYAIAMGLARLTKAILRNEQSILTVSTLMEGEYDLDDVYIGVPAIVSQKGVERAIEIDLNDEEMKKLHHSSNTLKDVMKPI-

General information: TITO was launched using: RESULT: Template: 4M1Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1660 -40666 -24.50 -134.21 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -24.50 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.568

(partial model without unconserved sides chains): PDB file : Tito_4M1Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M1Q-query.scw PDB file : Tito_Scwrl_4M1Q.pdb: