Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERNEVLKKVEAAMPNARFKHTLGVEKAAVELAEHYHMDVEKARITALLHDYAKYYDDDK-ARKIIEDEGYDPRLLSFHHSLWHAPVGAYLAEKEFGITDTEILEAIRLHTTGSATMSDFDKLIYLAD--YTEQGRTFPG------------VYKARRLALKSLDEAMLFALSNTITHLVKKQQL--------VFPDTLDAYNYFVNLNLEGDY------ 4S1B Chain:A ((5-220)) ---HPLLKKILMKAPGT-YHHSMMVANLAEACADKIGANSLLVRVGCFYHDIGKTLRPPYFVENQINPHDRLTPEQSRDIILSHTKDGAEILKEN--HMPQPIIDIALQHH-GTTLLKYFYFKAKETNPDVKEADYRYSGPKPQTKEIAIINISDSVEAAVRSSTEPTMAKITEIIDGIIKDRFLDGQFTECDITIQEIKIIRDTLIATLNGIYHQRIQY

General information: TITO was launched using: RESULT: Template: 4S1B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -35950 for 1379 contacts (-26.1/contact) + 2D Compatibility (PS) -19580 + (NN) -12345 + (LL) 524 1D Compatibility (HY) -2800 + (ID) 1650 Total energy: -71801.0 ( -52.07 by residue) QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_4S1B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4S1B-query.scw PDB file : Tito_Scwrl_4S1B.pdb: