Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISFIFAMDANRLIGKDNDLPWHLPNDLAYFKKITSG-------HSIIMGRKTFESIG---RPLPNRKNIVVTSAPDSE--FQGCTVVSSLKDVLDICSGPEECFVIGGAQLYTDLFP--YADRLYMTKIHHEFE----GDRHFPEFDESNWKLVSSEQG---T--------KDEKNPYDYEFLMYEKKNSSKAGGF 1AOE Chain:A ((8-192)) --IIVAALKPALGIGYKGKMPWRLRKEIRYFKDVTTRTTKPNTRNAVIMGRKTWESIPQKFRPLPDRLNIILSRSYENEIIDDNIIHASSIESSLNLVSDVERVFIIGGAEIYNELINNSLVSHLLITEIEHPSPESIEMDTFLKF-PLESWTKQP-KSELQKFVGDTVLEDDIKEGDFTYNYTLWTRK--------

General information: TITO was launched using: RESULT: Template: 1AOE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 633 12959 20.47 83.07 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 20.47 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.525

(partial model without unconserved sides chains): PDB file : Tito_1AOE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1AOE-query.scw PDB file : Tito_Scwrl_1AOE.pdb: