Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAQLFNLESRLDELENEINTQYCELDTNLDALKSNRIELESQLEKFESSLTNRLQGSISNNCRNDLLNLGYTHSQVDCMSDEE-----------------VYAALDKIDEEIHNTDQDYSTGFEDLEKQIIEMKRDYFIDRKERGSGNFDEAWEGEILDLEFEYTVLCLEKGLEPLNYIITWEG---------------------------- 4FZS Chain:A ((305-513)) SDIWFEEKLQEVECEEQRLR----KLHAVVETLVNHRKELALNTAQFAKSLAMLGSSEDNTALSRALSQLAEVEEKIEQLHQEQANNDFFLLAELLSDYIRLLAIVRAAFDQRMKTWQRWQDAQATLQKKREAEARLLWANKPDKLQQAKDEILEWESRVTQYERDFERISTVVRKEVIRFEKEKSKDFKNHVIKYLETLLYSQQQLAKYWEA

General information: TITO was launched using: RESULT: Template: 4FZS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 313 12211 39.01 74.46 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 39.01 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.256

(partial model without unconserved sides chains): PDB file : Tito_4FZS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FZS-query.scw PDB file : Tito_Scwrl_4FZS.pdb: