Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTIFEALKWASSYLTE-AGREENAAELLLLYDTGMERSKLLASLQEPIGEDELYRFKRHVEMHKEGVPVQYIIGKEFFYGREFMVNDDVLIPRPETEEVVFHLLEKYRSVFSEDGKLEVVDVGTGSGAIAVTLALENQSFSVSAVDISKEALQVASANAEKLGAN--VRFYQGDLLEPFIKAGKKADIIVSNPPYISEEEMADLSEIVRFHEPLHALTDGGDGLKFYKRFMEDIPLVMKDKVFVVFEIGWKQGAAVKDLILKAFKGAEVEVLKDINGKDRTICALIHKNK 1NV9 Chain:A ((15-284)) --KIWSLIRDCSGKLEGVTETSVLEVLLIVSRVLGIRKEDLFLK-DLGVSPTEEKRILELVEKRASGYPLHYILGEKEFMGLSFLVEEGVFVPRPETEELVELALELIRKY----GIKTVADIGTGSGAIGVSVAKFSDA-IVFATDVSSKAVEIARKNAERHGVSDRFFVRKGEFLEPFKEKFASIEMILSNPPYVKSSAL---------FEPPEALFGGEDGLDFYREFFGRY---DTSGKIVLMEIGEDQVEELKKIV------SDTVFLKDSAGKYRFLLLNRRSS-

General information: TITO was launched using: RESULT: Template: 1NV9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1325 3791 2.86 14.52 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 2.86 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_1NV9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NV9-query.scw PDB file : Tito_Scwrl_1NV9.pdb: