Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIAISGGGTGGHIYPALAFIKEVQRRHPNVEFLYIGTENGLEKKIVERENIPFRSIEITGFKRKLSFENVKTVMRFLKGVKKSKSYLAEFKPDAVIGTG-GYVCGPVVYAAAKMGIPTIVHEQNSLPGITNKFLSKYVNKVAICFEEAKSHFPSE--------------KVVFTGNPRASEVVSIKTGRSLAEF-GLSEDKKTVLIFGGSRGAAPINRAVIDMQDVLKTRDYQVLYITGEVHYEKVMNELKSKGAADNMVTKPFLHQMPEYLKAIDVIVARAGATTIAEITALGIPSVLIPSPYVTANHQEVNARSLGQHDAAIVLKETELSGEKLIEALDRIVLNEQTLKEMSERTKSLGVPDAAARLYSVLEELKK-- 1F0K Chain:A ((7-357)) KRLMVMAGGTGGHVFPGLAVAHHLMAQ--GWQVRWLGTADRMEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGMGG-YVSGPGGLAAWSLGIPVVLHEQNGIAG------------------LTNKWLAK-IATKVMQAFPGAFPNAEVVGNPVRTDVLALPL---PQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLG-DSVTIWHQSGKGSQQSVEQAYAEAGQPQHKVT-EFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPALFVPFQH-KDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGW--SRETLLTMAERARAASIPDATERVANEVSRVARAL

General information: TITO was launched using: RESULT: Template: 1F0K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1885 -208313 -110.51 -625.56 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -110.51 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.575

(partial model without unconserved sides chains): PDB file : Tito_1F0K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1F0K-query.scw PDB file : Tito_Scwrl_1F0K.pdb: