Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTIFEALKWASSYLTEAGREENAAELLLLYDTGMERSKLLASLQEPIGEDELYRFKRHVEMHKEGVPVQYIIGKEFFYGREFMVNDDVLIPRPETEEVVFHLLEKY--RSVFSEDGKLEVVDVGTGSGAIAVTLALENQSFSVSAVDISKEALQVASANAEKLGAN--VRFYQGDLLEPFIKA-----GKKADIIVSNPPYISEEEMADLSEIVRFHEPLHALTDGGDGLKFYKRFMEDIPLVMKDKVFVVFEIGWKQGAAVKDLILKAFKGAEVEVLKDINGKDRTICALIHKNK 2H00 Chain:A ((5-254)) --------VSLNFKDPEAVRALTCTLLREDF------------------------------------------------GLSIDIPLERLIPTVPLRLNYIHWVEDLIGHQDSDKSTLRRGIDIGTGASCIYPLLGATLNGWYFLATEVDDMCFNYAKKNVEQNNLSDLIKVVKVPQKTLLMDALKEESEIIYDFCMCNPPFFGITEIMAEG----------------GELEFVKRIIHDSLQLKKRLRWYSCMLGKKCSLAPLKEELRIQGVPKVTYTEFCQGRTMRWALAWSFYD

General information: TITO was launched using: RESULT: Template: 2H00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 978 -3196 -3.27 -14.79 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -3.27 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_2H00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H00-query.scw PDB file : Tito_Scwrl_2H00.pdb: