Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESENRTVIREFILLGLTQSQDIQLLVFVLVLIFYFIILPGNFLIIFTIKSDPGLTAPLYFFLGNLAFLDASYSFIVAPRMLVDFLSAKKIISYRGCITQLFFLHFLGGGEGLL-LVVMAFDRYIAICRPLHYPTVMNPRTCYAMMLALWLGGFVHSIIQVVLILRLPF-CGPNQLDNFFCDVPQVIKLAC------------TDTFVVELLMV-FNSGLMTL-LCFLGLLASYAVILCRIRGSSSEAKNKAMSTCITHIIVIFFMF------GPGIFIYTRPFRAF-PADKVVSLFHTVIFPLLNPVIYTLRNQEVKASMKKVFNKHIA 4PXF Chain:A ((53-317)) ---------------------------------------PINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFAT-LGGEIALWSLVVLAIERYVVVCKPMS-----NFRF-----------GENHAIMGVAFTWVMALACAAPPLVGWSRYIPEGMQCSCGIDYYTPHEETNNESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAVYNPVIYIMMNKQFRNCMVTTLCCGKN

General information: TITO was launched using: RESULT: Template: 4PXF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -183142 for 1731 contacts (-105.8/contact) + 2D Compatibility (PS) -24299 + (NN) -6609 + (LL) 6720 1D Compatibility (HY) -21600 + (ID) 3400 Total energy: -232330.0 ( -134.22 by residue) QMean score : 0.175

(partial model without unconserved sides chains): PDB file : Tito_4PXF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PXF-query.scw PDB file : Tito_Scwrl_4PXF.pdb: