Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKVALITTGGTIASKKTASGKLASGELSGEELAALCQLPTEIQIDIYSTFQLPSMHITLPDLISLKQLIESIFMDETYDGIVVTHGTDSLEETAYFLDLAVTDARPIVVTGSQRAPEEPGTDAYVNIRHAIYTACEINLRQAGTVVVFNERIFAARYVKKVHASNIQGFSAFGFGYLGIIDNDQVFLYQKPLEHECFDIRLD------LPEVVVIKCYIGADGLFIDAAIDSGVSGIVLEGVGRGQVAPKMMPAIIRALDAGIPVVITTSAEEGNVYTTYDYEGSTFDLYNRGVILGKDYDSKKARMKLMVLLASQEEINQTNFR 1NNS Chain:A ((81-318)) -------------------------------------------------------------------------------DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAADKASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARKHTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVFDTLATAAKTGTAVVRSSRVPTG--ATTQDAE---VDDAKYGFVASGTLNPQKARVLLQLALTQTKDPQQ----

General information: TITO was launched using: RESULT: Template: 1NNS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -187149 for 1924 contacts (-97.3/contact) + 2D Compatibility (PS) -25638 + (NN) -13637 + (LL) 6420 1D Compatibility (HY) -10400 + (ID) 3800 Total energy: -234204.0 ( -121.73 by residue) QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_1NNS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NNS-query.scw PDB file : Tito_Scwrl_1NNS.pdb: