Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKYMSLLTRIEEMMQSTSYQEGDRLPSIRQLSARYQVSKSTVIRALQELEKRHLIYSVPKSGYYIVKKSGKSKSGQPGPIDFATSAPDPDVFPYLDFQHCINKAIDTYKNDLFIYGTPKGLPSLIRVLRKLLATQQVFADERHIFITSGVQQALSLLCAMPFPNGKEKIAIEQPGYHLMVEQLETLGIPAIGVKRTEEGLDIAKVERLFQTESIKFFYTMPRFHNPLGCSLSERDKQELVRLAEAYDVYLVEDDYLGDLEENKKADPLYAYDLSSH-----VIYLKSFSKMMFPGLRVGAAVL-PEALTDTFYAYKKLNDIDCSMISQAALEIYLKSGMYGRHKEKIRDSYKERSLRLHQAIRTHRQLGSG-RFTFSSGQAPCMHTHLVLPQDLPASRVIHRLEKQGVLLEAIDRHYLSDYPKENLLKINISNVKTEDIERGVKLLMSHL 2ZYJ Chain:A ((31-390)) ----------------------------------------------------------------------------RPGILSFAGGLPAPELFPKEEAAEAAARILREKGEVALQYSPTEGYAPL-----RAFVAEWIGVRPEEVLITTGSQQALDLVGKV-FLDEGSPVLLEAPSYMGAIQAFRLQGPRFLTVPAGEEGPDLDALEEVLKRERPRFLYLIPSFQNPTGGLTPLPARKRLLQMVMERGLVVVEDDAYRELYFGEARLP-SLFELAREAGYPGVIYLGSFSKVLSPGLRVAFAVAHPEALQKLVQA-KQGADLHTPMLNQMLVHELLKEG-FSERLERVRRVYREKAQAMLHAL--DREVPKEVRYTRPKG---GMFVWMELPKGLSAEGLFRRALEENVAF-VPGGPFFANGGGENTLRLSYATLDREGIAEGVRRLGRAL

General information: TITO was launched using: RESULT: Template: 2ZYJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1992 -213597 -107.23 -605.09 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -107.23 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.575

(partial model without unconserved sides chains): PDB file : Tito_2ZYJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZYJ-query.scw PDB file : Tito_Scwrl_2ZYJ.pdb: