Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFQIDLNCD-LGESFGAYKIGLDQDILE-----YVTSANIACGFHAGDPSVMRKTVALAA--ERGVKMGAHPGL-----------------PDLLGF--GR--RNMAISPEEAYDLVVYQIGALSGFLKAEGLHMQHVKPHGALYNMAAVD-QKLSDAIAKAVYKVDPGLILFGLAESELVKAGERIGLQTANEVFADRTYQSDGTLTPRSQPDALIESDDAAVTQVIKMVKEGAVKSQQGHDVSLKADTVCIHGDGAHALTFAQKIRKQLKAAGIEVTAISEQRST 2I5I Chain:A ((3-151)) NKKLIINADDFGY-----TPAVTQGIIEAHKRGVVTSTTALPTS----PY-FLEAMESARISAPTLAIGVHLTLTLNQAKPILPREMVPSLVDEAGYFWHQSIFEEKVNLEEVYNEWDAQIISFMK----SGRRPDHIDSHHNVHG----KNKKLLGVALALARKYQ------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2I5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 485 -53667 -110.65 -450.98 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -110.65 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.272

(partial model without unconserved sides chains): PDB file : Tito_2I5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I5I-query.scw PDB file : Tito_Scwrl_2I5I.pdb: