Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISKKVVLPLVFSAPFIFFFVLCIVVVMTISRENQVGDDFIGGGDGEYETVGIAPEVERFRAVFEKYARQEGVFDQVNIIMALTMQESGGRSLDIMQSSESIGLPPNSITDPERSIEVGIKHFKKVFKQAGGDVRLTLQAYNFGSGFIDYVKKNGGKYTKKLALDFSRLQAFKMGWKSYGDPSYVDHVMRYVKGSDKNVKPVKGSMDFYETVMKEALKYEGQPYAWGGSNPE------------TGFDCSGLVQWSFAKAGITLPRTAQEQHGATKKISEKEATAGDLVFFGGTYEGKAITHVGIYVGNGRMFNSNDSGIQYSDLKSGYWRDHLVSFGRIK 4Q4G Chain:X ((1082-1189)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EYVIRRGMSQIGVPYSWGGGNAAGPSKGIDSGAGTVGFDASGLVLYSFAGVGIKLPHYSGSQYNLGRKIPSSQMRRGDVIFYG----PNGSQHVTIYLGNGQMLEAPDVGLK--------------------

General information: TITO was launched using: RESULT: Template: 4Q4G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 402 -33536 -83.42 -349.33 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain X : 0.57 3D Compatibility (PKB) : -83.42 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.613

(partial model without unconserved sides chains): PDB file : Tito_4Q4G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q4G-query.scw PDB file : Tito_Scwrl_4Q4G.pdb: