Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKTTNGWINGFIGVLIFSGSLPATRLAVSDFDPLFLTVCRAAIAGVLAGGLLLIFRQQHPAKRDLISLLVVAFGVVIGFPLLTALALQHVTSAHAIVFIGLLPLATAVFGVLRGGERPRPVFWIFSAAGSLLVAGFALIQGGGSSPL--GDAYMLASIVVCGLGYAEGAKLSRRLGNWQVISWALVLSLPLMLPLSFFFTPDSWSAIGVPALLSLAYVSLFSMLIGFVFWYRGLAQGGIAAVGQLQLLQPFFGLLLASVILHEKVGWALVAVNIAVIMCVAAARRFAK 4X14 Chain:A ((193-244)) ----------------------------------------------------------------------------------------------------------TRYFGTLTGGENVPPVLHITNTATTVLLDEF------GVGPLCKGDNLYLSAVDVCGM-----------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4X14.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 43 -3078 -71.58 -61.56 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.44 3D Compatibility (PKB) : -71.58 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.44 QMean score : -0.046

(partial model without unconserved sides chains): PDB file : Tito_4X14.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X14-query.scw PDB file : Tito_Scwrl_4X14.pdb: