Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFGTIFNTVMIIAGSIIGGIFKKGIKDEYQDILMQAMGFAAVALGINAITQHLPDSKYPILFIVSLAIGGLLGQIINLELRFNKLVNKFSKSNLAEGLSTAVLLFCIGSLSILGPVEAALHGDYTYLLTNGMLDGITSIVLASTFGFGIAAAALVLFSWQGSIYLFAQVMESAINTDLINEITIVGGILILSSGLSILGIKKFKTLNLLPSLLIPPVVIFVIHAFGLRF 2MMM Chain:A ((27-41)) -------------------------------------------------------------------------GLKKLGKKLEGAGKR---------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2MMM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4 223 55.75 14.87 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.37 3D Compatibility (PKB) : 55.75 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.37 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_2MMM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MMM-query.scw PDB file : Tito_Scwrl_2MMM.pdb: