Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRILSILVFAIMLAGCSSNASTEKQHAGGEKTVKAEPQSTSSQKDSTDDYQPNSQVTDDRTLHKVGQTFSDDKGKAVLKDIKQVNKTYKIGDVELTVKEMKLIHLRPDYSMIDYFHELTHDEEFDFVKVFVDIKNTST-KKVNVAP-I---ALMKTNTG----ETFDWNK----D---IYLEELNGELEGGAEKSGNLGFIVNASSGHAHDKAADAEKKTKEIKWIEITTSDVFDHNHKKISDAQKIKIKF 3QFG Chain:A ((62-166)) --------------------------------------------------------------------------------------DTIVSDKFEAKIKEPFIINEK--------------DEKKKYIAFKMEITAKKDDKDLNPSSISHDYINITQD-DKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFIYELRGD----------------------------------------------

General information: TITO was launched using: RESULT: Template: 3QFG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 349 -20293 -58.15 -228.01 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -58.15 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.297

(partial model without unconserved sides chains): PDB file : Tito_3QFG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QFG-query.scw PDB file : Tito_Scwrl_3QFG.pdb: