Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLSDNNFVSETLENVQYLLPGAKVIKLRGYSRAHKVYTIAKSPV-EKWKVAAGL--SGSEIAIL---IRKGHWIGASIP-AGGIVIDIDDSKQGELV--KGLLDAQNFHCHCIRTLM-GGSLFLRITNMGKRKLNK 1RO2 Chain:A ((15-115)) --------------------GFNIIPID---------PESKKPVLKEWQKYSHEMPSDEEKQRFLKMIEEGYNYAIPGGQKGMVIMDFESKEKLKAWIGESALEELCRKTLCTNTVHGGIHIYVLSNDIP------

General information: TITO was launched using: RESULT: Template: 1RO2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 251 -35788 -142.58 -393.27 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -142.58 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.138

(partial model without unconserved sides chains): PDB file : Tito_1RO2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RO2-query.scw PDB file : Tito_Scwrl_1RO2.pdb: