Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNILRAMVILLIICGTYVLFIQYGSVPEKKSNDSEPQVSNEEAQSGKRIHMPTSGLLSFMGKHADEVTKKLGEPERIDPSAYDYDWWVYNQGKDQYIQIGVLNNKVVTLFASGNDINAKPFKIGESTGEVFKTTQVAPFVNVEYKGNSYRFEFSEEDINTRPTVKVGKMYVQLYMDKFEGKLSSIRAFDAQTFVKQRPYEVVYRGELIKPKAVSDEKWKKIQTTSEKQILDLTNVIRVKH-----------------GLAKLEWDQPTAEVAFGHSEDMKENNYFSHVSKKYGSLKDRLEEGHVDFQQAGENIAYN------YVDGPAAVEGWLN-----------------SEGHRKALLNSDYTHLGVGVDRKYYTQNFIKRW 1QNX Chain:A ((41-177)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LTKQEKQDILKEHNDFRQKIARGLETRGNPGPQPPAKNMKNLVWNDELAYVAQVWANQCQY----GHDTCRD---V--------AKYQVGQNVALTGSTAAKYDDPVKLVKMWEDEVKDYNPKKKFSGNDFLKTGHYTQMVWANTKEVGCGS-------------

General information: TITO was launched using: RESULT: Template: 1QNX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 339 -12657 -37.34 -130.48 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -37.34 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.248

(partial model without unconserved sides chains): PDB file : Tito_1QNX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QNX-query.scw PDB file : Tito_Scwrl_1QNX.pdb: