Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELIIILLALGLLMFTAYRGFSVILFAPICALFAVLLTDPSHVLPFFSSIFMEKMAGFIKLYFPVFLLGAIFGKVVEMAGLAASIAKTIVRLVGAKRAILAIVLMGAVLTYSGVSLFVVVFAVYPFAKNMFQEANIPKRLIPGTIALGAFTFTMDALPG-TPQIQNVIP----TSFFK-------TDIYAAPWLGLMGAVIVLAAGMLYLESRRKKAQASGEGYGGFDSQNAPAPESIESAAEPDKSPIRHALA---FVPLILVGAVNKYFTIYLPKWYPNGFD-FSSIGLKEFGRLDISSAAAIWSVEIALVIGIITTILF---DW--RSV--FAQLKEGLNEGIGGALLASMNTGAEYGFGGIIAALPGFHK-LSSGISHT--FT-DPLV---NGAVTTTALAGITGSASGGMGIALSAMSEQYLQAIQAYNIPPEVMHRVISMASGG-MDTLPHNGAVITLLAVTGLT-----HRQSYRDIFAITLIKTAA-VFAVIAIYSLTGLV 4R0C Chain:A ((72-488)) -------------------------------------------------TLVGNFTGFAP-LG-VVLVAMLGLGVAEQSGLLSVSLASLVRRSSGGAL-VFTVAFAGVL--SSLTVDAGYVVLIPLAGLVFQLAGRPPIAGIATAFAAVS-GGFSANLLVGPV--DATLAGLSTEAAHIIDPDRTVAATGNYWFIIASTFLVTGLVTLITRT-LTE-PRLAHANTVADA-----SVDAPQIHSR-----AMKWTGLTLAILLAGLALLV-----LP-ND-APLRHPDTG-----S-VLGSPF----IHGLVVIVALIAGICGAVYGRVSGQFRNSGAVITAMEVTMASMAGYLVLMFFAAQFVAWFNYSQLGLLLAVKGAAWLGALTVPKVVLLLLFVVLTALINLMIGSASAKWSILAPVFIPMLM----LLGISPEASQAAYRVGDSSTNIITPLMPYFVLVLGFARRYQPETGIGTLIALMLPYSLTLLLGWS-VLLGVWIGFGW-

General information: TITO was launched using: RESULT: Template: 4R0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1860 -216164 -116.22 -568.85 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -116.22 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_4R0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R0C-query.scw PDB file : Tito_Scwrl_4R0C.pdb: