Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRLHYAWIIVSVTFLILLAVQGVRLSFGAFVEPWER------QFSIDRSTISLISTVSFIVYGISQPVIGRLVDKW-GARAVLAWSALLTGVSIFLTYLVT--SPWQLFLLYGLGVSLGVGGASNVAASVLVVNWF-S--KKRGLAFGIMEAGFGAGQMLLVPGSLMLIHWFSWKLTVVVLGLLLIVIVFPAALL-MLRNNPSEKNTEPIGGLAASE-KETAPKTT----------------------------------------------ALSVAGMFRMRQFWF----LIFPFLICGFTTVGLMDTHLIPFSHDH-GF----STTVTSAAVSLLAGFNIAGILLSGIVADRWSSRKI------LCILYAVRALSIVILIYS-----------HEPYLLLAFAILFGLVDFATVAPTQMLATQYFQNYSIGLMIGWLSLAHQIGSALGAYVPGVIYTVT-GEYTLAFYLSIGMLVLASVMNVMLREPAAVTRDSAAVVDK 4W6V Chain:A ((33-491)) -----------------------SFYGIRPLLILFMAATVFDGGMGLPREQASAIVGIFAGSMYLAALPGGLLADNWLGQQRAVWYGSILIALGHLSIALSAFFGNDLFFIGL-VFIVLGTGLF-KTCISVMVGTLYKPGDARRDGGFSLFYMGINMGSFIAPLLSGWLLRTHGWHWGFGIGGIGM-LVALLIFRGFAIPAMKRYDAEVGLDSSWNKPT-----GRWVTAIMAVVVVIIALISQGVIPINPVMIASLLVYVIAASVTLYFIYLFAFAKMSRKDRARLLVCFILLVSAAFFWSAF-EQKPTSFNLFANDYTDRMVMGFEIPTVWFQSINALFIILLAPVFSWA---------PSSITKFVIGILCAAAGFAVMMYAAQHVLSSGGAGVSPLWLVMSILLLTLGELCLSPIGLATMTLLAPDRMRGQVMGLWFCASSLGNLAAGLIGGHVKADQLDMLPTLFARCSIALVICAAVL-------------------

General information: TITO was launched using: RESULT: Template: 4W6V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1701 -289961 -170.46 -814.50 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -170.46 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.200

(partial model without unconserved sides chains): PDB file : Tito_4W6V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4W6V-query.scw PDB file : Tito_Scwrl_4W6V.pdb: