Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLITVPLAGELKFYPLNEEFRVSFGAPVFFFFLSLLRHVPAVLPGFLTGAAVFIFRVFLELWGGGHNGLTPILYDQASGFFFYMTYACLFSILKANRFRERPIMLGFIGFMIEVVSDCVELTVQFLIFHTVVTPEKITDIAVIAISHTFIVMSFYSVLKLYETQSREKQTRQQHEHMLMIVSNLYEETVHLKKTLKTTEKVTNDSYQLYREMKGKDVQLSGRILRLAGEIHEVKKDNQRIFAGLSKLISNESLRDYMRASDLLQLVIRMNEKYAEALGKQIDFYCSIEGEHDEYHVFIVLSIINNLTANAVEAMDEEGMVSLRLR-KPNESMVEFQVEDNGPGISEKIGDIVFDPGF-TSKYDEFGTPSTGIGLSYVKEIVTELEGDITFDN-QQRGVVFAIRLPVRHLIQKG 3SL2 Chain:A ((34-153)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DLYVEIDQDK-------ITQVLDNIISNALKYSPEGGHVTFSIDVNEEEELLYISVKDEGIGIPKKDVEKVFDRFYRVD------LGGTGLGLAIAKEMVQAHGGDIWADSIEGKGTTITFTLPYKE-----

General information: TITO was launched using: RESULT: Template: 3SL2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 464 -52616 -113.40 -474.01 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -113.40 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.678

(partial model without unconserved sides chains): PDB file : Tito_3SL2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SL2-query.scw PDB file : Tito_Scwrl_3SL2.pdb: