Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------MSGYSNGGGYGGISSFALIVVLFILLIIVGTAFVGGF 1HA8 Chain:A ((1-51)) GECEQCFSDGGDCTTCFNNGTGPCANCLAGYPAGCSNS--DCTAFLSQCY-----------GGC

General information: TITO was launched using: RESULT: Template: 1HA8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 48 -9125 -190.10 -380.21 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -190.10 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.144

(partial model without unconserved sides chains): PDB file : Tito_1HA8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HA8-query.scw PDB file : Tito_Scwrl_1HA8.pdb: