Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAEFTHLVNERRSASNFLSGHPITKEDLNEMFELVALAPSAFNLQHTKYVTVLDQDVKEKLKQA-ANGQYKVVSSSAVLLVLGDKQAYQQAADIYEGLKVLGILNKQEYDHMVQDTVSFYENRGEQFKRDEAIRNASLSAMMFMLSAKEKGWDTCPMIGFDAEAVKRILNIDDQFEVVMMITIG---------KEKTESRRPRGYRKPVNEFVEYM 4EO3 Chain:A ((149-320)) ---------EWRRARRALKKDRVPREELELLIKAAHLAPSCMNNQPWRFVVVDEEELLKKIHEALPGGNYWMKNAPA----------------------LIAVHSKKDFDCALPDN------------RDYFLFDTGLAVGNLLVQATQMGLVAHPVAGYDPVKVKEILKIPEDHVLITLIAVGYLGDESELSEKHRELERSERVRKELSEIVRW-

General information: TITO was launched using: RESULT: Template: 4EO3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 671 -84739 -126.29 -523.08 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -126.29 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_4EO3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EO3-query.scw PDB file : Tito_Scwrl_4EO3.pdb: