Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MISIMMKVSLAVFMLAGGIIKVSRVPFQVEHWR-------------HYQYPLWFLTVTGILEIAGAL----------AMTAGIWNRYAAIGAGVLFVVLMAGAI-------------HAHMFRARQSVIMAIQAMICLIVSIMIIMGSYT-- 1Z23 Chain:A ((546-708)) GSGREPLELEVAVETLARLQQGVSTTVAHLLDLVGSASGPGGWRSTSEPQEPPVQDLKAAVAAVHGAVHELLEFARSAVSSATHTSDRTLHAKLSRQLQKMEDVYQTLVVHGQVLDSGRGGPGFTLDDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKA

General information: TITO was launched using: RESULT: Template: 1Z23.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 338 -86537 -256.03 -759.09 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -256.03 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_1Z23.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Z23-query.scw PDB file : Tito_Scwrl_1Z23.pdb: