Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MRNPVVWGMIYFAVGC---IFTYLAA---------SSPGSMWSFYSILLMVFAAYNISISFKMFAFSFKIKKNQK 1E0F Chain:I ((1-57)) IRFGMGKVPCPDGEVGYTCDCGEKICLYGQSCNDGQCSGDPKPSSEFE-----------------------EFEIDEEEK

General information: TITO was launched using: RESULT: Template: 1E0F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 69 -10797 -156.47 -269.91 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain I : 0.56 3D Compatibility (PKB) : -156.47 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.128

(partial model without unconserved sides chains): PDB file : Tito_1E0F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E0F-query.scw PDB file : Tito_Scwrl_1E0F.pdb: